Activating Compound | Comment | Organism | Structure |
---|---|---|---|
catalase | - |
Hyoscyamus niger |
General Stability | Organism |
---|---|
rapid loss of enzyme activity during purification | Hyoscyamus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 100% inhibition | Hyoscyamus niger | |
MgSO4 | 24% inhibition | Hyoscyamus niger | |
MnSO4 | 100% inhibition | Hyoscyamus niger | |
additional information | no marked effect on enzyme activity by addition of NAD+, NADH, NADP+, NADPH, ATP + MgSO4, FAD, FMN, pyrroloquinoline quinone, acetyl-CoA, 6,7-dimethyl-5,6,7,8-tetrahydrofolate, phenazine methosulfate, 2,6-dichlorophenolindophenol, cytochrome c and H2O2 | Hyoscyamus niger | |
Pyridine 2,4-dicarboxylate | - |
Hyoscyamus niger | |
ZnSO4 | 100% inhibition | Hyoscyamus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
(6S)-6-hydroxyhyoscyamine | - |
Hyoscyamus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Hyoscyamus niger | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required as co-factor | Hyoscyamus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | Hyoscyamus niger | alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species | scopolamine + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hyoscyamus niger | - |
- |
- |
Oxidation Stability | Organism |
---|---|
requires molecular oxygen | Hyoscyamus niger |
Purification (Comment) | Organism |
---|---|
partial, could not be separated from hyoscyamine 6beta-hydroxylase | Hyoscyamus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Hyoscyamus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000198 | - |
- |
Hyoscyamus niger |
Storage Stability | Organism |
---|---|
-20°C, enzyme is stored in small portions until assayed | Hyoscyamus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | - |
Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? | |
(6S)-6-hydroxyhyoscyamine + 2-oxoglutarate + O2 | alkaloid metabolism, scopolamine is formed by oxidative transformation of hyoscyamine in several solanaceous species | Hyoscyamus niger | scopolamine + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hyosOH epoxidase | - |
Hyoscyamus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Hyoscyamus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Hyoscyamus niger | |
ascorbate | - |
Hyoscyamus niger |